By Oscar; Latner, A. L. Bodansky
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Extra resources for Advances in Clinical Chemistry, Vol. 19
I 4 z 4 25C 2 1. L& 0 MANUAL I I 250 500 u IU/ 1000 500 liter FIG. 11. Correlation between lactate dehydrogenase activities in serum (IUAiter) determined at 35°C with the LKB 8600 reaction-rate analyzer and at 25OC by manual spectrophotometry. The regression equation is: y = 2 . 996; n = 101). Points shown as 0 are referred to the outer pair of axes. 28 D. MOSS 0-5 6-10 O/o 11-15 16-20 Difference FIG. 12. Histogram of the agreement between duplicate estimations of lactate dehydrogenase activity in serum by a manual spectrophotoinetric method at 25°C (open bars) and by the LKB 8600 reaction-rate analyzer at 35°C (filled bars).
4 (Fig. 7). This method is applied in the LKB Produkter AB 8200 calculator available for use with the LKB 8600 reaction rate analyzer. The parallel fast analyzers (A3) which employ centrifugation as a means of mixing sample and reagents and initiating the reaction provide examples of the collection of large numbers of readings of absorbance during enzymic reactions. In these instruments the individual reaction mixtures enter measurement cuvettes by centrifugal action. The cuvettes pass through the light beam of the photometer once in every revolution of the reaction rotor.
W. 7. , Cambridge CB1 2PX, England) is made up of the AC 1 automatic chemistry unit, the SP 30 spectrophotometer, and the DR 16 digital printer (Fig. 20). A strip-chart recorder can also be added to monitor the reaction progress-curves. The AC 1 unit performs the functions of sample dilution, reagent addition, mixing, preincubation, initiation of the enzymic reaction, and transfer of the completed reaction mixtures to the cuvette of the spectrophotometer. The serum or other samples are loaded in disposable cups into racks which also carry the disposable reaction tubes.
Advances in Clinical Chemistry, Vol. 19 by Oscar; Latner, A. L. Bodansky